FastQCFastQC Report
Mon 27 Feb 2023
SRR3127093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896194
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108321.2086668734671286No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52680.5878191552275511No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG35070.39132152190262376No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG27850.310758608069235No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC23660.26400533812991384No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15040.1678208066557018No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT12750.14226830351464081No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACA11200.12497294112658643No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT10360.11559997054209246No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT10290.11481888966005128No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT10240.11426097474430759No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9420.10511117012611108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT750.087.729128
CGTTTTT31100.085.993431
ATAGCGG1850.083.988451
CGCTACG9200.082.246053
ACGGGAT4150.081.5382464
AGGGATG28200.081.496085
TCCGCTA9450.081.2144241
CGTAGGG2500.080.885762
AGGGAAT19150.080.2520145
TACGGGA2350.079.9961553
GGATGGC21300.079.87417
ACCGATG650.079.534649
CGAAGGG7550.079.1043552
AAGGGCA10350.079.0107044
GCACGGG1250.079.004692
GGGCGAT3300.078.329576
CGAGGGA5400.078.329573
GCGATTG301.4562938E-678.329578
GGAATGC12450.077.763337
ATGGCAC20600.077.112799
Produced by FastQC (version 0.11.5)