FastQCFastQC Report
Mon 27 Feb 2023
SRR3127092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3450832
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413381.197914010302443No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61290.17760934174714968No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57770.16740890312828907No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG35920.10409083954246395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126600.085.6419751
AGGGATG70200.075.460915
CGTAGGG10150.074.551662
ATAGCGG6450.073.624571
ACGGGAT10000.071.919324
AGTAGGG53900.071.415522
TAGGGCG6750.071.031424
TAGGGAT21550.070.018354
ATAGGGC21650.069.470773
TAAGGGA32650.068.666553
TAGGGCA19700.068.242344
GTACGGG7450.068.134172
ATAGGGA31050.067.9664843
AGGGCAT30450.067.766095
GTAGGGA27050.067.7653053
GAGGGAT52250.067.652784
TAGCGGG17300.067.647332
AAGGGAT65400.067.346644
AGTAAGG22950.066.99251
TAGAGGG63850.066.985062
Produced by FastQC (version 0.11.5)