FastQCFastQC Report
Mon 27 Feb 2023
SRR3127091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754278
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298581.0840590528624925No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49190.17859489855417643No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45460.1650523295034125No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG32090.11650966242332836No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG29950.10873993111806433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91000.086.6098561
AGGGATG57450.076.154645
GTACGGG6750.074.021422
CGTAGGG7750.072.9852142
TAGGGCG5250.072.7232364
AGATAGG15700.071.8009951
GAGGGAT40750.071.252574
ACGGGAT8700.070.974044
CGAAGGG26400.069.990242
GTAGGGA22450.069.705853
ATAGGGC15650.069.57373
AGTAGGG45300.069.507812
GAATAGG16000.068.39121
TAAGGGA23850.068.380963
AGGGACT39900.068.311185
ATAGCGG4400.067.533631
AAGAGGG92750.067.4391862
TAGGGCA14500.067.289894
CGAGGGA18150.067.262123
ATAGGGA23300.067.162933
Produced by FastQC (version 0.11.5)