FastQCFastQC Report
Mon 27 Feb 2023
SRR3127090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868867
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313361.0922778922829117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51810.18059394178956362No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46470.16198032184831154No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG31040.10819602302930041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97200.086.088411
CGTAGGG6600.080.505832
ACGGGAT7300.078.540754
AGGGATG56900.077.059735
GAATAGG17750.072.914441
TAGGGCG5250.072.5076454
ATAGCGG5200.071.499491
AGTAGGG43000.071.297112
GTACGGG7300.070.8537142
ATAGGGC17050.070.0111853
AGGGAAT36000.069.710285
GTAGGGA22800.069.669063
AGATAGG17250.069.298491
TAGGGCA16600.068.228694
TAAGGGA26150.067.5731663
GAGGGAT41550.067.5245064
AAGGGAT53600.067.5124744
GTAAGGG46400.067.0861362
TAGAGGG54600.067.000762
TAAGGGT13850.066.845863
Produced by FastQC (version 0.11.5)