FastQCFastQC Report
Mon 27 Feb 2023
SRR3127089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574622
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357891.0011967698962296No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57690.1613876935799086No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG37240.10417884744177146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119350.083.0840451
ATAGGGA33750.071.156753
AGTAGGG59150.070.474472
ATAGGGC21050.069.658093
GAATAGG20450.068.745041
CGTAGGG9950.068.486952
ACGGGAT9600.067.562744
TAGAGGG68650.067.362392
TAGGGCA18850.067.071864
AATAGGG53500.066.760992
ACGGGTA4750.066.2949754
GTAGGGC16750.065.935933
GGTAAGG19400.065.194961
AAGGGTA26900.065.171244
TAAGGGA33850.065.1153263
GATAGGG51300.065.043512
ATAAGGG51800.064.960032
GTAGGGA29750.064.926783
GTAGGGT13700.064.835113
CGAAGGG38200.064.83512
Produced by FastQC (version 0.11.5)