FastQCFastQC Report
Mon 27 Feb 2023
SRR3127088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839100
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262320.9239547744003382No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43700.15392201754076995No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG35390.12465217850727342No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG29470.10380050015850092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86150.085.172161
ACGGGTA3050.078.7992554
TAGGGCA14650.073.019694
CGTAGGG7650.070.841572
ATAGGGA25050.070.73463
AATAGGG40900.069.362532
ATAGGGC17200.069.317653
GAATAGG17000.068.23851
AGTAAGG18050.067.404021
AGGGAAT28900.066.994785
TAGGGAT18600.066.887164
TAAGGGA26850.066.6946643
AGTAGGG45150.066.4864962
AGGGATG41350.066.484745
TAGCGGG17650.066.482352
CGAAGGG27950.065.9244842
TAGAGGG50350.065.890822
AGGGCAT26550.065.667585
AAGGGAT47650.065.272884
AAGAGGG102000.065.1896442
Produced by FastQC (version 0.11.5)