FastQCFastQC Report
Mon 27 Feb 2023
SRR3127087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975005
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275420.9257799566723417No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47160.15852074198194627No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG35140.11811744854210329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89850.082.3387451
CGTAGGG8750.074.688352
TAGACGG4250.071.977091
ATAGGGC17200.071.589783
ACGGGAT9450.069.626494
ATAGGGA28600.069.510843
AGTAAGG18900.068.725381
TAAGGGA27700.068.71533
AGTAGGG50400.068.37852
ATGCGGG16200.068.202292
ACGGGTA3200.067.559444
GAATAGG16900.067.390471
TAGGGCA14450.067.3256764
TAGGGAT20050.066.8049554
AATAGGG44450.066.531142
ATAAGGG38800.066.282942
AAGGGAT48450.065.767924
AGGGATG43300.065.44975
GTAGGGA22700.065.4243553
GTAGGGC14250.065.302313
Produced by FastQC (version 0.11.5)