FastQCFastQC Report
Mon 27 Feb 2023
SRR3127086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679253
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262090.9782204218862497No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53310.19897337056261577No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32630.12178767738619684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82950.083.493621
ATAGGGC18650.078.120193
ACGGGTA2300.073.565024
GAATAGG17550.071.800021
ACGGGAT6800.071.190984
TAGGGTA8700.070.22974
AGGGTAC10600.070.05515
ATAGGGA26600.069.0836943
AGTAGGG43800.068.994962
AGGGATG47300.068.6602555
CGTAGGG7500.068.3039252
TAGGGCA16350.068.128254
GAGGGAT33200.067.810054
TAGAGGG49900.067.436172
TAGGGTC7600.067.407714
GTAGGGT9950.067.0726853
AATAGGG42900.067.046262
GGTAAGG18400.066.6944351
AGGGAAT30900.065.707335
GCAAGGG46150.064.870642
Produced by FastQC (version 0.11.5)