FastQCFastQC Report
Mon 27 Feb 2023
SRR3127085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109813
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196320.9305090071963723No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42180.1999229315583893No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23540.11157386934292281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63550.085.116921
ATAGGGC14000.079.4386753
AGATAGG13350.072.770281
ATAGGGA20850.071.873543
TAGGGCA11950.070.982724
AGTAGGG34750.070.115612
ACGGGAT5350.069.5859154
AGGGAAT24900.069.075475
GAGGGAT25300.068.917454
AGGGATG35650.068.810245
AGACGGG13800.068.6429442
GAATAGG12900.068.362821
ACGGGTA1800.068.068924
CGTAGGG4950.067.597732
TAGGGTA6400.067.005344
GTAGGGC11300.065.891083
GGTAAGG13900.065.4802861
AGAGGGC32200.065.272013
AAGAGGG74750.065.19112
GTAAGGG33300.065.101732
Produced by FastQC (version 0.11.5)