FastQCFastQC Report
Mon 27 Feb 2023
SRR3127084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195116
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205030.9340280878094825No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44550.20295055022149172No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24540.11179363641830317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63900.083.291221
GAATAGG13950.075.9006961
TAGGGTA7700.072.6305854
ATAGGGC12600.070.8673863
ATAGGGA20550.070.4372253
AGGGATG38950.069.9816065
AGTAGGG35750.069.962792
GGTAAGG14850.069.71621
GAGGGAT27500.069.554474
GTAGGGC9900.067.883493
AATAGGG34100.067.281712
AAGAGGG81350.066.635132
TAGAGGG42800.066.5672152
AGAGGGC34000.065.656553
GTAGGGT9550.065.4502943
TAAGGGA22700.065.422113
GTAAGGG35400.065.076522
CGTAGGG4850.064.947832
TAGGGCA11250.064.7504044
CGAAGGG21750.064.631392
Produced by FastQC (version 0.11.5)