FastQCFastQC Report
Mon 27 Feb 2023
SRR3127083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176058
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325031.023375517701503No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG63840.20100388594918606No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC59710.18800034508185934TruSeq Adapter, Index 21 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59320.18677240780867352No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG57190.18006598116281253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96450.082.771971
CGTAGGG8200.072.7880252
ATAGCGG6300.072.390081
AGTAGGG45650.070.4182742
TAGGGCA15900.070.058754
GGTAAGG16500.069.811991
ATAGGGC17900.069.578743
AGTAAGG19750.069.274561
GTAGGGA23050.068.91753
AGAGGGC47450.068.73963
AGGGCAT30300.068.5620655
ATAGGGA25950.068.46023
TAAGGGA27950.067.9333953
AGGGATG44350.067.5071955
TAGGGTA12050.066.699134
AGATAGG18650.066.301711
AGGGTAC12550.066.287835
TAGGGAT18850.066.0762944
AAGAGGG102100.066.007532
GAGGGAT38950.066.007164
Produced by FastQC (version 0.11.5)