FastQCFastQC Report
Mon 27 Feb 2023
SRR3127082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524122
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234920.9306998631603385No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG60590.24004386475772566No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC50660.20070345252725502TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG49570.19638511926127183No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46490.18418285645464047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68700.083.930071
TACGGGT2500.077.276983
CGTAGGG6600.075.677752
GTACGGG5550.073.863952
GTAGGGA18250.071.519293
AGTAAGG15800.070.71951
AGTAGGG38850.070.5891952
ATAGGGA21250.070.513853
TAGCGGG11250.070.366032
ACGGGTA3300.069.9662254
AGGGCAT20200.069.793215
TAGGGCA13650.069.730864
AGGGATG32450.068.499875
AGAGGGC35050.067.8905943
TAGGGAT14100.067.8395844
TAGGGCG4800.067.735154
CGAAGGG24500.067.69912
AAGAGGG78850.067.6473852
ATAAGGG31300.067.594022
ATAGGGC15750.067.314433
Produced by FastQC (version 0.11.5)