FastQCFastQC Report
Mon 27 Feb 2023
SRR3127081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677033
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249120.9305824769436911No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG60420.22569762868070734No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50140.1872969066873662No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG49930.1865124561408096No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC49720.18572800559425304TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74900.083.549891
ATAGGGA22550.074.6121143
AGGGATG36500.070.94665
TAAGGGA22750.070.857453
TAGGGAT14450.070.252014
CGTAGGG7300.070.196332
GTAGGGA19250.070.068693
AGTAGGG38550.068.7805252
AGGGAAT29600.068.431865
AGGGCAT20700.068.1120155
TAGGGTA9050.068.029234
TAGAGGG44450.067.9006652
AGAGGGC37850.067.79533
GTACGGG6450.067.7850652
GAGGGAT31000.067.463854
AGATAGG17050.067.33511
AAGGGAT43000.067.107764
ATAGGGC16050.067.055333
AAGGGCG10800.067.0146564
GAATAGG17900.066.503361
Produced by FastQC (version 0.11.5)