FastQCFastQC Report
Mon 27 Feb 2023
SRR3127080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733920
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256970.9399324047521508No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77600.283841516942705No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG57780.2113448820740914No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG49990.1828509978346111No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC46200.16898811962310528No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45380.1659887633873705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79950.086.274221
GACCGAT1650.082.6039358
AGGGATG46550.075.218265
AGGGTAC15000.074.2580955
AAGGGAT51100.073.12084
AGGGCAT26900.072.507435
ACGGGTA2750.071.781294
TAAGGGA27750.070.963943
CGAAGGG25300.070.589782
GCTACGA18150.070.4347464
GTAGGGC11150.070.3928153
TAGGGCA15300.070.345894
CGTAGGG7650.070.036132
GAGGGTA17000.069.6700744
AGTAGGG41550.069.5637362
AGAGGGC40550.069.1942753
AGGGAAT35400.069.170545
TAGGGCG5300.069.1693044
AAGGGTA19450.068.8683854
CGCTACG18600.068.7294163
Produced by FastQC (version 0.11.5)