FastQCFastQC Report
Mon 27 Feb 2023
SRR3127079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182777
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189030.8660069260396275No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58120.26626632037995634No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG53560.24537550102461222No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG42660.19543911265328526No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC37770.17303645768669912No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35250.16149153120085102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57950.085.0065461
AGGGTAC13150.078.6215065
ACGGGTA2100.078.532374
GACCGAT1400.077.204788
AGGGATG37450.076.796965
AGGGCAT24000.076.561415
TAGACGG2650.076.491311
AAGGGTA16900.075.837184
CGAAGGG19450.073.64852
AGGGTAT15850.073.530385
GTAGGGC10500.072.698543
AGGGAAT29000.071.139525
AAGGGAT41750.070.876634
TAGGGCA13100.070.499294
TAAGGGA20550.070.163223
AAGGGCG10300.069.535454
ACGGGAT5700.069.439154
AGTAGGG32850.069.4256442
GTAGGGA16650.069.3348853
GGTAAGG14650.069.1815641
Produced by FastQC (version 0.11.5)