FastQCFastQC Report
Mon 27 Feb 2023
SRR3127078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288291
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195390.8538686731713756No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62540.27330440053297417No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG52380.22890445314865987No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG40890.17869230792761934No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38020.16615019680626283No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC36240.15837146586688494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63450.084.542121
GACCGAT750.081.46118
TACGGGT2100.080.5659263
AGGGCAT24000.076.761425
TAGACGG2800.075.623091
AAGGGTA17400.074.816734
CGAAGGG20250.074.756832
CTACGAC13200.074.4115755
GTACGGG5450.074.186012
CGCTACG13350.073.9275363
CACCGAT1150.073.560199
CGTAGGG6650.073.524442
GGTAAGG12800.073.154841
AGGGATG39400.072.8808065
TAGGGCA14050.072.5857854
GTAGGGT11850.072.577323
AGGGTAT17200.072.4078455
AGAGGGC32700.072.2916953
AAGGGCG10450.071.95684
GCTACGA13700.071.695824
Produced by FastQC (version 0.11.5)