FastQCFastQC Report
Mon 27 Feb 2023
SRR3127077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449234
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448631.3006655970572019No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54150.15699137837560456No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41880.12141826272152019No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG41370.11993967356230398No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC41070.11906991523335327TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG40040.11608374497062246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139300.084.013191
ATAGGGC21000.073.859213
AGGGATG63200.073.402375
ATAGCGG5300.071.8592761
ATAGGGA33650.070.816243
AGTAGGG58250.070.762162
AGAGGGC51150.070.295223
TAGGGCA18350.070.1848454
AGGGAAT43600.070.1785665
GTAGGGA27950.070.1236043
ACGGGAT10150.069.463094
GAATAGG20850.069.2318651
GGTAAGG19500.068.720131
AGGGCAT31000.068.0760965
AAGAGGG124300.067.872012
CGAAGGG35550.067.6905062
AGGGTAC14000.067.144745
ATGAGGG50500.066.2652742
TATAGGG28250.066.0494842
AAGGGAT64050.066.0468754
Produced by FastQC (version 0.11.5)