FastQCFastQC Report
Mon 27 Feb 2023
SRR3127076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738220
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326871.1937316943123635No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41720.1523617532557647No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG37490.13691376149469364No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC34260.12511777724215TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG33880.1237300143889096No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32840.11993192658004105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101900.084.255661
ACGGGTA2650.080.028934
AGGGTAC10850.076.229745
ACGGGAT7450.074.645934
ATAGGGA24850.074.5326463
AGGGATG49100.074.0799945
ATAGCGG4250.072.108721
GAATAGG15450.072.018961
ATAGGGC16300.071.415053
TAGGGCA13400.071.395634
AGGGAAT35850.070.916885
AGTAGGG44200.070.372352
AGAGGGC38900.069.056673
AGGGCAT24000.068.9243855
AAGGGTA17900.068.7175754
ACTAGGG13800.068.301672
CGAAGGG27100.067.6488952
ATGAGGG41700.067.471242
GAGGGTA14750.067.417214
GAGGGAT37400.067.415944
Produced by FastQC (version 0.11.5)