FastQCFastQC Report
Mon 27 Feb 2023
SRR3127075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882496
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344541.195283532050001No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43630.15136187526366038No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG37820.13120573280934303No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36300.12593252514487444No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC34450.11951447634272519TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG34130.11840432736073181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106100.084.18791
ACGGGAT9000.078.32894
ATAGGGA25500.074.642833
AGGGATG52000.073.117015
TAGGGCA14450.072.8540044
GAATAGG18450.071.676831
TAGGGTC8050.070.641964
AGTAGGG44100.069.7306062
ATAGGGC18200.069.721323
TAACGGG8700.069.7195742
GATAGGG37600.069.6548842
AGAGGGC42100.069.323863
AGGGAAT34150.068.947785
AGGGTAC11050.068.475765
CGAAGGG26300.067.401542
ATGAGGG41050.067.237132
GTAGGGC13800.067.09043
AGGGCAT27200.066.867545
AAGGGAT52800.066.490554
AAGAGGG99250.066.467822
Produced by FastQC (version 0.11.5)