FastQCFastQC Report
Mon 27 Feb 2023
SRR3127074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059570
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288360.9424853819327552No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT64680.21140225587255723No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG44590.1457394339727478No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG38650.12632494108649256No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC37020.12099739505878276No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33040.10798903113836257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87200.083.945681
ATAGGGC19200.075.635793
TAGGGCA18200.075.1464
AGGGAAT36700.071.842445
AGTAGGG50900.071.5573042
GACCGAT2450.070.9750448
TAAGGGA26200.069.957353
TATAGGG29300.068.9716952
TAGAGGG54150.068.911572
CGTAGGG10150.068.527642
GTAGGGA28700.067.793573
AGGGATG48300.067.724595
ATAAGGG36750.067.3943252
CGAAGGG30100.066.982432
ATAGGGA30950.066.965023
AAGGGTA20800.066.430614
AGAGGGA75600.066.081753
AGGGTAC13150.065.76225
AAGAGGG109350.065.6281742
GAGGGAT38550.065.224934
Produced by FastQC (version 0.11.5)