FastQCFastQC Report
Mon 27 Feb 2023
SRR3127072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560978
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222630.8693163314952336No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51950.20285219162366877No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG40730.1590408039428687No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG31800.1241713126782034No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC28870.11273037097546328No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26040.10167990509875524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70050.084.29231
ATAGGGC16000.076.66513
AGGGAAT29800.073.808575
ATAGGGA22850.072.604863
TAGGGCA15950.072.485584
CGTAGGG8000.072.291532
TAAGGGA22600.071.328473
CGAAGGG23250.070.1744162
AGTAGGG43500.070.0419162
ATAACGG2500.069.632831
CGGGTAT2100.069.377655
GTAGGGA22400.068.188723
AGGGATG38350.067.524825
ATAGAGG19250.067.4575651
TAGAGGG48250.067.4343262
GAGGGAT29850.067.229634
TAGCGGG12950.067.169832
TATAGGG23800.067.169822
TAGGGAT18400.066.6653064
AATAGGG34450.066.331422