FastQCFastQC Report
Mon 27 Feb 2023
SRR3127071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262427
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386541.1848234458579456No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55990.17162069833286692No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47580.14584234375205943No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC36630.1122783743513648No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG36290.11123620543846652No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG35170.10780317843127218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121750.083.677961
ACGGGAT8650.075.528934
ATAGCGG6450.073.61781
ATAGGGC20850.073.2586063
TAGGGCA16600.072.2015844
GAATAGG23200.071.330591
AGGGATG51000.071.3288965
GGTAAGG16850.071.147791
CGTAGGG9050.071.146472
ATAGGGA31450.069.6380543
AGTAGGG57650.069.620952
TAAGGGA29650.068.476323
TAGCGGG17000.068.009182
GTAGGGA28400.067.849533
TAGGGCG5900.067.714484
AGGGTAC13500.067.5402455
AGGGCAT27700.066.8514945
AATAGGG52450.066.756292
AAGAGGG114600.066.724342
GTAGGGC15500.066.707143
Produced by FastQC (version 0.11.5)