FastQCFastQC Report
Mon 27 Feb 2023
SRR3127070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570869
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286871.1158483765606104No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43530.16932017928568122No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36110.14045834307387892No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG36080.14034165101372337No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC31910.12412145465210403No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG31460.12237107374977099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91200.084.374891
ATAGGGC17750.076.196363
CGTAGGG6500.073.229182
AGTAGGG43350.072.947352
TAGGGCG5200.072.499894
TAGGGCA14000.072.370424
TAGGGTA8400.071.2484054
GAATAGG18300.071.1124951
AGGGATG37450.070.396685
ACGGGAT7700.070.381384
AGTACGG2550.070.263751
GGTAAGG14850.070.1703641
GTAGGGT10450.069.447263
ACGGGTA2250.069.1165544
ATAGAGG19650.068.866381
TAGCGGG11950.068.621062
AGGGCAT23500.068.591045
GTAGGGC14500.068.574893
TATAGGG20650.068.0099262
TAGGGTC7100.067.70064
Produced by FastQC (version 0.11.5)