FastQCFastQC Report
Mon 27 Feb 2023
SRR3127069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741820
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300821.0971544448577952No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46640.17010598799337667No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39620.14450255669591733No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG36070.1315549525497662No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC32180.11736729617553304No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG31290.11412127710790643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94750.082.950141
ATAGCGG6250.074.5478441
GGTAAGG15950.071.996111
TAGGGCA15300.071.263344
AGGGATG40300.070.670345
ACGGGTA2400.070.4954154
AGTAGGG46450.070.2556152
TAAGGGA22400.070.07583
ATAGGGC18150.069.653873
ACGGGAT6300.069.376444
GAATAGG18650.069.143551
AGGGCAT23050.069.1191565
AGTAAGG18550.069.0088651
TAGCGGG14700.068.774662
ATAGGGA25500.068.744553
AAGAGGG88600.067.084262
ATAGAGG19050.066.2094041
CGTAGGG7750.066.135112
TAACGGG7850.065.891642
GAGGGAT34500.065.795724
Produced by FastQC (version 0.11.5)