FastQCFastQC Report
Mon 27 Feb 2023
SRR3127068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886114
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT866983.00397004414933No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT74380.2577167776463438No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT61200.2120498358692692No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT54770.18977074363659924No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT45350.1571317002724078No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG36100.12508168423007546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT265950.088.952751
AGGGATG44700.073.81025
ATAGGGC18100.071.9263463
GTAGGGC12950.071.133323
AGGGAAT30550.069.382855
TAGGGCA13950.069.069934
AGTAGGG40850.068.7999952
GAATAGG16900.068.446851
ACGGGAT8100.067.890694
GAGGGAT34350.067.45744
ATAGCGG6650.067.1749041
ATAGGGA25300.065.9469453
AGGGACT47550.065.827975
AGAGGGC40150.065.201333
TGTAGGG18550.065.113112
GTAGGGA18750.064.921013
AGGGCAT24150.064.416585
AAGAGGG99200.064.2432252
GTTTTTT372250.064.174822
GTAGGGT10550.064.150033
Produced by FastQC (version 0.11.5)