FastQCFastQC Report
Mon 27 Feb 2023
SRR3127067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294543
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656592.8615284176413343No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59250.2582213538817969No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT56670.24697728480137437No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT50080.21825696881688422No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT42910.18700891637245412No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG31360.13667209548916712No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC23470.10228616330136328No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC23410.10202467332274881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT202650.089.58151
ATAGGGC12200.075.361263
GAATAGG13050.072.3018341
TAGGGCA10050.071.779224
AGGGATG36250.071.429255
TAGGGTC5400.070.723654
AGAGGGC34800.068.826853
GGTAAGG13450.067.3455351
AGATAGG12550.067.288241
AGGGCAT18150.067.058785
AGGGAAT24550.066.996035
AGTAGGG32400.066.940082
ACGGGAT6350.066.825494
TAGAGGG39900.066.646852
GATAGGG31800.066.275632
AGGGACT34000.066.204745
GTAGGGA15050.066.102723
GTAGGGT8150.065.950893
ACGGGTA2300.065.598754
GAGGGAT28800.065.321154