FastQCFastQC Report
Mon 27 Feb 2023
SRR3127066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392106
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676202.8267978091271875No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT63140.26395151385431914No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT54280.2269130214129307No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT48350.2021231500610759No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT44260.18502524553677804No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG30500.12750271100026503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT203950.089.6228561
ATAGGGC14550.073.323773
AGGGATG38650.072.7166065
TAGGGCA11000.070.9246754
ATAGGGA20100.070.380513
AGGGACT36500.069.145415
GATAGGG35400.067.874772
ATAGCGG5150.067.621961
GAATAGG14900.067.591291
AGATAGG14100.067.087291
ACGGGAT7500.067.050874
AGAGGGC36450.066.532543
AGTAGGG34150.066.090842
TAGCGGG14450.065.7316062
AGGGAAT26850.065.640055
GACCGAT2100.064.902388
GAGGGAT28400.064.7053764
AAGGGTA13400.063.833484
GTAGGGA17650.063.374453
GTTTTTT290700.063.373862
Produced by FastQC (version 0.11.5)