FastQCFastQC Report
Mon 27 Feb 2023
SRR3127065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060612
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208341.011058850477431No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34410.16698922456047038No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG32190.15621572620173038No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG26320.12772904360452136No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC23800.11549966708919487TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61150.082.820491
ATAGGGC9700.074.13393
GAATAGG13750.072.844281
AATAGGG29250.072.468262
AGATAGG12400.068.639711
TAGTAGG9450.068.1721951
TAGGGCA10150.068.077124
TAGAGGG37150.067.305412
ATAGGGA18300.067.289513
ACGGGAT5200.065.988684
AAGAGGG69800.065.651762
ATGCGGG10400.065.528742
GGATAGG9700.065.445621
AGTAGGG32700.065.109992
GTAGGGA15450.065.100233
AAGGGAC21650.064.917714
TAAGGGA19000.064.5630653
GATAGGG28300.063.939832
CGTAGGG5450.063.8164142
AAGGGTA15050.063.7152754
Produced by FastQC (version 0.11.5)