FastQCFastQC Report
Mon 27 Feb 2023
SRR3127064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634733
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153470.938807744139257No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG30250.18504550896079056No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27340.1672444368591079No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG24980.15280782855671232No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC20900.1278496243729098TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44900.083.7884141
ACGGGAT3250.081.1980444
ATAGGGC8100.079.121553
ATAGCGG3100.072.997341
AGATAGG10150.072.457941
TAGAGGG28900.071.260872
GTAGGGA12200.070.68583
ATAGGGA14000.070.34923
GAATAGG9450.070.3421
TAGGGCA8500.069.2998054
TAGGGAT9500.068.949664
TAGTAGG6300.068.845371
AGTAGGG26050.068.564462
CGAAGGG16150.068.282732
ATAGGGT7900.067.405023
AATAGGG21250.067.197212
GATAGGG21650.067.044072
GTACGGG3250.066.7024842
AAGAGGG53550.065.3878252
AGAGGGC22900.065.23263
Produced by FastQC (version 0.11.5)