FastQCFastQC Report
Mon 27 Feb 2023
SRR3127063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696911
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159130.9377627936880603No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG29970.17661503755942415No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29050.17119342145816724No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG23060.1358939861902009No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC20710.12204529288807721TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49600.084.144321
ACGGGAT3400.078.784544
ATAGGGC8550.074.7511443
GAATAGG9600.074.009681
ATAACGG2100.071.6990661
TAGGGCA8550.069.254744
ATAGCGG4150.069.161531
GTAGGGA11400.067.605823
AGATAGG9350.067.433551
TAAGGGT7550.067.223613
ATAGGGT7450.066.864353
AATAGGG23750.066.71192
ATAGGGA13850.066.165243
TAAGGGA13900.065.927233
TAGAGGG33150.065.806912
TAGCGGG9600.065.625192
CGTAGGG3800.065.573642
AATGCGG4250.065.319961
AAGAGGG56050.065.259092
AGGGACT24500.065.216565
Produced by FastQC (version 0.11.5)