FastQCFastQC Report
Mon 27 Feb 2023
SRR3127062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407040
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49211.2089720911949686No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG16360.4019261006289308TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG14300.3513168238993711TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC12190.2994791666666667TruSeq Adapter, Index 22 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6100.1498624213836478No Hit
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT5320.1306996855345912No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT4900.1203812893081761No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4900.1203812893081761No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT4890.12013561320754716No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG4690.11522209119496855No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC4660.11448506289308176No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4340.10662342767295598TruSeq Adapter, Index 23 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT4080.10023584905660378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG301.3238605E-893.999648
TACGGGT201.8365003E-593.9996263
GCGATAA254.915564E-793.9996268
TTACGGG409.094947E-1293.9996262
GTTGCGA201.8365003E-593.9996267
TAGCGGG1500.087.7329942
TAGGGCA1650.085.4542164
CGTTTTT19850.084.0860751
TAGACGG401.031367E-982.281
CTATAGG401.031367E-982.281
CGACGAT353.8584403E-880.571115
AGGGCAT3150.080.5711065
CGTAGGG650.079.538152
GTAGGGC1850.078.756453
GATACGT301.4544803E-678.333039
TTGACGG301.4544803E-678.333036
ATAGGGC2350.077.9996953
ACGGGAT551.8189894E-1276.908794
AGGGAAC2700.076.592295
GAATAGG2100.076.122991
Produced by FastQC (version 0.11.5)