FastQCFastQC Report
Mon 27 Feb 2023
SRR3127060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343488
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34911.01633827091485No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG14810.43116498975218936TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG12590.3665339109372089TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC11160.32490217998882054TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT5220.15197037451089995No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5000.1455654928265325No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4770.13886948015651202No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT4510.13130007452953232No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT4480.13042668157257314No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4310.12547745481647102TruSeq Adapter, Index 23 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT4090.11907257313210359No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATG3890.11324995341904229No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC3780.11004751257685857No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG3600.10480715483510342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG850.094.018642
TACGGGT156.900606E-493.9912643
GCCGATG201.8369015E-593.9912648
GTCGTTG156.900606E-493.9912647
GTATAGG1000.089.395811
TACGAGG700.087.379371
TAACGGG550.085.471492
ATGGGCG550.085.44664
TAGGGCA1100.085.44664
CGTTTTT12550.084.353361
TAGCGGG900.083.572122
CGAGGGA1650.082.598393
GGCCGAT700.080.563947
ATAGGGC1650.079.750173
AGGGCAC1600.079.305135
TATAGGG2400.078.348872
GCTAGGG850.077.427122
AGGACGG551.8189894E-1276.991611
GAGTAGG2100.076.176891
TAAGGGA3300.075.477843
Produced by FastQC (version 0.11.5)