FastQCFastQC Report
Mon 27 Feb 2023
SRR3127059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069733
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363981.185705727501382No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50750.16532382458018335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111700.082.593721
ATAGGGC18850.077.547223
ATAGCGG6200.075.083531
AGTAGGG48000.073.0479052
TAGGGCA15650.072.984454
GGTAAGG18850.072.341551
TATAGGG26500.069.526492
GAATAGG15200.068.986241
AGGGATG46400.068.0697865
ATGCGGG17200.067.496092
AGAGGGC46900.066.845193
GAGGGAT41300.066.580074
AGGGAAT34500.066.073265
TAGGGTA9600.065.610294
GTAGGGC12950.064.968143
TAGAGGG61250.064.7659152
AGGGCAT27900.064.688945
AGATAGG18650.064.292831
AAGAGGG115000.064.248772
AAGGGGC100100.063.9090654
Produced by FastQC (version 0.11.5)