FastQCFastQC Report
Mon 27 Feb 2023
SRR3127057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469902
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281391.1392759712733542No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40530.16409557950072515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86000.085.025851
GGTAAGG15150.075.16251
ATAGGGC15650.074.7763753
ATAGCGG4700.073.084191
TAGGGCA13700.073.069894
AGAGGGC40600.072.349153
GAATAGG14750.069.863531
TAAGGGA21100.069.271943
AGTAGGG40500.068.4967352
AGGGATG37000.068.464665
AGGGAAT24750.067.980955
TAGGGTA6800.067.732424
ATAGGGA21400.067.422373
TATAGGG21750.067.231692
AAGAGGG95050.066.830722
GTAGGGT10250.066.026473
ATGTAGG7350.065.93991
GTAGGGC11250.065.5883263
ATAAGGG33300.065.5158842
GAGGGAT32500.065.363384
Produced by FastQC (version 0.11.5)