FastQCFastQC Report
Mon 27 Feb 2023
SRR3127055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399207
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45531.1405110631827597No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG20940.5245399003524488TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC16940.4243412565410927No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG16810.4210848006172237TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT6690.16758223177449294No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT6570.16457627246015225No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5700.1427830674311823No Hit
GATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC5620.1407790945549552No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5480.13727214202155774No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC4970.12449681493560984No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG4900.1227433386689111No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4740.11873539291645688No Hit
GGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4720.1182343996974001No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4640.11623042682117297No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT4430.11096999802107678No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT4370.10946701836390645No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4220.10570956922098058No Hit
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG4220.10570956922098058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG156.770836E-494.444061
TGTACGG156.770836E-494.444061
TAGACGG450.094.444051
AGGGCGA850.088.4543155
CGTAGGG700.087.6318742
ATAGCGG600.086.573721
CGTTTTT16050.086.205941
TAAGCGG500.084.999651
AGGGTCC950.084.089795
CAGGGCG401.0022632E-982.576194
GTAGGGC1450.081.355853
TAGGGCG353.7536665E-880.890964
GTACGGG1000.080.2168662
AACGAGG1300.079.91421
GCGTAGG301.4141951E-678.7033841
TAGGGTC1750.078.194594
TAGCGGG1150.077.960132
GTAGGGT1150.077.960133
GAGTAGG1700.077.777451
GTGTTAG255.56E-575.186175
Produced by FastQC (version 0.11.5)