FastQCFastQC Report
Mon 27 Feb 2023
SRR3127054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411261
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45751.1124322510522515No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG20060.4877681083302331TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC16480.40071876496920444No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG15630.38005062478572TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT6640.16145464802157267No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT6380.15513262867133037No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT6220.1512421552250274No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5680.13811180734375494No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG5570.13543710684942167No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5430.13203294258390658No Hit
GATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC5410.1315466334031187No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC5130.12473830487208853No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4610.11209426617160391No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT4420.10747432895411915No Hit
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG4380.10650171059254342No Hit
GGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4250.10334070091742227No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT4210.10236808255584652No Hit
GAAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT4140.10066600042308899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG450.094.054141
ACGGGTA254.916492E-793.996964
ATAGAGG1400.090.695061
AGGGCGA1100.089.724375
CGTAGGG800.088.165032
ATCGAGG650.086.819211
CGTTTTT16150.086.774411
GCACGGG800.082.287362
GGCGATG401.033186E-982.247347
AAGGGTC1150.081.736494
CGGTCGG353.842979E-880.6178361
GCGTAGG353.842979E-880.6178361
CACGGGG1400.080.568823
TGCGGGC353.8593498E-880.568823
TGACGGG1200.078.368922
GTACGTC301.4547513E-678.33088
GTAAAGG2050.077.996121
GGTAAGG1950.077.172621
ACCGGGA551.8189894E-1276.906613
ACGGGCC551.8189894E-1276.906614
Produced by FastQC (version 0.11.5)