FastQCFastQC Report
Mon 27 Feb 2023
SRR3127053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979415
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453321.139162414576012No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT79930.20085866892495505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT155650.082.8921361
CGTAGGG10850.076.235862
ATAGGGC25000.072.379153
ACGGGAT7900.071.98834
AGTAGGG62550.070.1771852
TAGGGCA24050.069.377234
TAGAGGG71100.069.075342
AGTAAGG26100.067.55681
TAGGGCG6850.067.2417764
AGGGATG54750.067.128955
GTAGGGC22000.067.0810243
TAGGGTA14750.066.5974964
AAGAGGG144350.066.353492
AATAGGG60100.066.234662
ATAGGGA38500.066.043383
AGAGGGC59850.066.042673
GTACGGG7850.065.856612
GTAGGGT16100.065.390533
GAATAGG26350.065.309871
AGGGACT56300.065.197355
Produced by FastQC (version 0.11.5)