FastQCFastQC Report
Mon 27 Feb 2023
SRR3127052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167829
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337911.0666926781717068No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61690.19473904683617707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108200.084.11131
CGTAGGG7700.074.6746752
ATAGGGC19900.071.996443
ACGGGTA2750.070.265424
ATAGCGG5850.070.1273961
AGTAGGG50850.069.3289952
TAGGGTC10200.069.307714
AGGGATG43300.068.157385
AGTAAGG18150.068.068941
TAGAGGG56300.067.724272
GTAGGGA24500.067.327493
AGAGGGC47400.067.015013
AGGGACT47750.066.6279455
ACGGGAT6300.066.5794144
GGATAGG18050.066.3560941
AAGAGGG112350.065.861382
ATAGGGT13900.065.777513
TGTAGGG22750.065.465112
TAGGGCA17900.065.296394
GGTAAGG18650.064.726991
Produced by FastQC (version 0.11.5)