FastQCFastQC Report
Mon 27 Feb 2023
SRR3127051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286653
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347591.057580462555676No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT65460.199169185186267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118650.083.68691
CGTAGGG8450.077.89522
ACGGGTA3300.075.478434
GTACGGG6000.072.090162
ACGGGAT6500.071.578534
ATAGCGG7250.070.750781
AGTAGGG51000.070.2464142
TAGGGTA11400.070.0817644
AGGGATG44050.069.9872365
ATAGGGC18900.069.375053
TAGGGCA17500.068.748444
AAGAGGG114500.068.449382
TAGAGGG56150.067.9063642
AGTAAGG19550.067.3990861
GTAGGGA24450.067.082233
CGAAGGG31300.066.8428652
AGAGGGC49050.066.398033
AATAGGG49600.066.162682
GTAGGGT13400.065.934693
GAATAGG21750.065.774431
Produced by FastQC (version 0.11.5)