FastQCFastQC Report
Mon 27 Feb 2023
SRR3127050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532701
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187780.7414219049149504No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62950.2485488812141662No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG39680.15667068477487078Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG35710.14099571958948173RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC29960.118292684371349RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72150.084.251181
CGGGATG13450.080.723625
ACGGGAT11800.079.265094
CGAAGGG20850.077.0882342
AGGGATG68350.075.986185
ATAGCGG4400.075.861161
GGGCGAT10450.075.5605856
CTACGAC12600.074.978445
CGCTACG12500.074.818863
AAGGGAT74450.074.242664
CGTAGGG7600.074.205432
AAGGGCG13900.074.052594
GTAGGGC16150.073.914433
TAAGGGA37400.073.5109943
TAGGGCA22550.073.368054
GCGGGAT17200.073.234764
AGGGATT54400.072.921315
TCCGCTA12850.072.805431
ATAGGGC20950.072.682363
GCTACGA12950.072.589054
Produced by FastQC (version 0.11.5)