FastQCFastQC Report
Mon 27 Feb 2023
SRR3127049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003630
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137960.6885502812395502No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45360.22638910377664537No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG38120.19025468774174872Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG33230.1658489840938696RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC27790.13869826265328428RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2050.085.0928044
ACGGGAT11050.084.9054954
CGTTTTT52400.084.801561
CGAAGGG16450.082.2547452
CGGGATG11350.081.5650565
TACGCGG1050.080.866271
AGCGGGA19000.079.155673
GCGGGAT15000.078.576684
ATAGCGG3950.077.62481
TAGCGGG9750.077.367812
AGGGATG53350.076.6334845
TAAGGGA30800.076.382663
AAGCGGG23700.076.1895452
AAGGGAT59100.075.226214
CGTAGGG5900.075.113982
CGCTACG8850.075.113983
AAGGGAC25550.073.2562264
ATAGGGC16400.073.018833
AGGGATT41200.072.542695
TCCGCTA9300.072.533281
Produced by FastQC (version 0.11.5)