FastQCFastQC Report
Mon 27 Feb 2023
SRR3127048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091587
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143290.6850778858350143No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47910.22906051720535653No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG37900.18120212068634964Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG31900.15251576912650538RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC26680.12755864326944086RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT10450.084.099614
CGTTTTT53400.083.5204541
TAGGGCG5350.081.695454
CGGGATG11800.081.6470645
ACGGGTA2250.081.46124
CGAAGGG15700.079.350542
AGGGATG58600.076.109225
TAAGGGA31500.075.344153
AAGGGCG11250.075.1949544
GCGGGAT15800.074.956994
AGGGCGA11300.074.862235
AAGGGAT63450.074.217244
TAGCGGG11250.073.964692
ATAGGGC16850.073.3541263
CGTAGGG6350.073.293452
TAGGGCA17700.073.283224
CTACGAC9000.073.10625
AAGGGCA46000.072.436444
AGGGATT45250.072.2868655
CGCTACG9150.071.394113
Produced by FastQC (version 0.11.5)