FastQCFastQC Report
Mon 27 Feb 2023
SRR3127044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938769
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303651.0332557611707487No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT76400.2599727981341848No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC68600.23343107267022348No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG46030.15663020809053044No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG38500.13100723466186012No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC34540.11753220481092594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87950.082.860051
TAGGGCG5600.073.8627854
AGGGATG64300.072.734955
TAAGGGA27350.071.829963
AGTAGGG44100.071.18972
CGTAGGG8800.071.031062
GGGCGAT12000.070.895886
TAGGGAT23300.070.6062554
ATGCGGG14250.070.249632
GAGGGAT44650.070.215894
ATAGGGA29950.069.517413
AGGGTAC13700.069.3045655
GGTAAGG17050.069.2147451
AGACGGG17150.069.058272
TAGGGCA18600.068.989144
AAGGGCG16600.068.8064654
AAGGGAT59000.068.752714
CTACGAC14300.068.368865
ATAGGGC22700.067.910153
GAATAGG17000.067.758911
Produced by FastQC (version 0.11.5)