FastQCFastQC Report
Mon 27 Feb 2023
SRR3127042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439341
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239040.9799367944047184No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT63710.26117709660109023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54160.2220271786519392No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG44110.18082752677874886No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG35350.14491618842957996No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC34110.13983284829796244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71150.084.915081
TAGGGCA14050.074.592934
AGGGATG53850.074.095385
ATAGGGC16300.073.234433
TAGGGTA8550.072.556614
TAAGGGA21800.070.9265063
AGGGTAC12350.070.780755
AAGGGCG13150.070.763384
TAGCGGG12450.070.623012
AGACGGG13000.070.5285952
GAATAGG14800.070.2627641
AGGGAAT32600.069.918695
AGTAGGG36250.069.9124762
ATAAGGG29100.069.801492
ACGGGAT5800.069.685054
TAGGGCG5550.069.436854
GGGCGAT9900.069.3085566
AGGGCAT23750.068.8628165
TACGACC12350.068.49756
ATAGGGA23500.068.395483
Produced by FastQC (version 0.11.5)