FastQCFastQC Report
Mon 27 Feb 2023
SRR3127041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3664901
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445031.2143029238716134No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57930.15806702554857555No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57080.15574772688266342No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG41370.11288163036327584RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC39410.10753360049834906TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG38870.10606016369882841Illumina PCR Primer Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142550.084.370661
ATAGGGC21900.075.967463
ACGGGAT10900.074.163164
AGATAGG22350.072.9978561
ACGGGTA4350.072.388874
TATAGGG29050.071.3445742
TAGGGTA13550.071.105114
GTAGGGC18800.070.995193
AGGGATG58800.069.778755
AGTAGGG58200.069.768482
CGTAGGG11800.069.300392
GAATAGG24700.069.288721
ATAGGGA37600.068.7453463
TAAGGGA32050.068.332353
TAGGGCA18550.068.154524
TAGAGGG62600.067.417162
AGGGAAT43750.067.355925
AAGGGTA27850.067.333924
AGGGCAT31900.067.330495
ATAAGGG49600.067.2736742
Produced by FastQC (version 0.11.5)