FastQCFastQC Report
Mon 27 Feb 2023
SRR3127040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891302
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326721.1300099401584476No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45400.15702268389811924No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44510.1539444859098081No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG36420.12596401206100227Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG35390.12240160315318149RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC32370.11195648188947402TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106500.084.748691
ACGGGAT7700.076.466214
ATAGGGC17650.073.6571043
ACGGGTA3800.071.894334
AGATAGG18900.071.8198551
AGGGATG45400.071.216865
GAATAGG18300.071.083991
CGTAGGG8100.070.946772
AGGGAAT36700.070.940455
TATAGGG20950.070.824632
ATAGGGA28400.070.488923
GGTAAGG16000.069.519371
AGTAGGG43100.069.508452
ATAGCGG5300.069.363751
AGGGCAT25800.068.852915
GTAGGGC14200.068.66453
ATAAGGG37050.068.017922
TAGAGGG49100.067.921862
ATGAGGG46450.067.4362952
CGAAGGG30300.067.313642
Produced by FastQC (version 0.11.5)