FastQCFastQC Report
Mon 27 Feb 2023
SRR3127039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067905
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341221.1122247918367747No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49180.16030483342867527No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46740.1523515232707662No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG35570.11594231242492842Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG35480.11564895262402193RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC33340.10867350846913448TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112250.084.569951
ATAGGGC17800.075.51013
ACGGGAT8200.075.078644
TAGGGCA15800.074.0629964
AGGGATG48450.072.651915
GTAGGGC14450.071.875923
TATAGGG22300.070.63362
ATAGGGA29850.070.218173
AGATAGG18850.068.412541
AGTAGGG44400.068.410222
CGTAGGG7950.068.0145952
AGGGAAT37600.067.8689355
AGAGGGC44300.067.788523
CGAAGGG32600.067.6435852
AGGGTAC13850.067.1853265
ACGGGTA3850.067.136854
AGGGCAT26000.067.059385
AAGAGGG107250.066.768872
TAGAGGG50800.066.733352
TAGGGTA10850.066.7037054
Produced by FastQC (version 0.11.5)