FastQCFastQC Report
Mon 27 Feb 2023
SRR3127038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938489
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286710.975705541181199No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT71950.2448537326496713No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG38370.1305773137146336No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG37630.1280590126422117No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC29750.10124250933047563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89100.084.958771
GAATAGG16350.074.192831
TAGACGG3450.070.8670351
ATAGGGA26550.069.392343
ATAGAGG20200.068.8971
AGTAGGG46100.068.815372
CGTAGGG7900.068.415322
TAGCGGG14800.067.957082
AGGGATG35650.067.7643055
TGTAGGG22450.067.6192
GTAGGGA23400.067.4859543
TATAGGG22150.067.473922
AGAGGGC40150.066.957693
GTACGGG7400.066.686852
TAGAGGG50550.066.383432
AGGGAAT34650.066.193295
GGTAAGG16900.065.6576461
ATAGGGC17050.065.605853
AAGAGGG100250.065.586722
GAGGGAT29750.065.5640644
Produced by FastQC (version 0.11.5)