FastQCFastQC Report
Mon 27 Feb 2023
SRR3127036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432948
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226680.9317091857285893No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59780.2457101425924434No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG35960.1478042276283751No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG35360.14533808367462026No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC29680.12199192091240749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68300.083.324021
GAATAGG12250.073.009961
GTACGGG5450.070.749972
CGTAGGG6250.070.722362
GGATAGG12500.070.420021
ATAGAGG17650.069.874891
AGTAGGG38850.069.3541642
ATTGCGG2250.069.0392461
AGAGGGC32050.068.918793
GGTAAGG15300.068.608511
GTAGGGA17650.068.4315263
ATGTAGG8150.067.576061
ATAGGGA21850.067.107493
AGGGATG29650.066.889145
AGGGCAT21600.066.796315
TAGAGGG41550.066.658142
TAGACGG3250.066.625281
AGGGACT42450.066.537225
ATAGGGC12300.066.4832153
AAGAGGG84000.066.223842
Produced by FastQC (version 0.11.5)