FastQCFastQC Report
Mon 27 Feb 2023
SRR3127035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475762
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532301.531462741119789No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51430.14796755358968766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT157550.085.886231
ATAGGGC24600.073.5532463
GAATAGG23750.072.8509751
TAGGGCA19900.071.092024
CGTAGGG10250.070.149572
ATAGGGA32150.069.729033
TAAGGGA31750.069.719353
AGTAGGG56000.069.6542362
AGATAGG19650.069.388351
AGTAAGG21850.067.781361
AGGGATG54500.067.526265
TAGAGGG65800.067.4222952
TATAGGG28450.067.396192
GTAGGGC17300.067.10073
GTAGGGA26950.066.9651263
AGAGGGC49650.065.881913
AATAGGG57300.065.859492
TGTAGGG26850.064.936232
AGGGCAT27650.064.424575
ATGTAGG10900.064.2703861
Produced by FastQC (version 0.11.5)